Abstract: The resonance characteristics of the centrifuge baseplate have a significant impact on its operational stability and lifespan. In this study, the finite element simulation method is used to investigate the differences in natural frequencies of three different structural designs of centrifuge baseplates: solid baseplate, three-hole baseplate, and six-hole baseplate. The geometric parameters of the three baseplates are identical, with a diameter of 2000 mm, spindle height of 600 mm, spindle diameter of 320 mm, and hole diameter of 300 mm; and the Q235 low-carbon steel is selected as the material. The modal of three kinds of baseplates is calculated by finite element analysis software (Abaqus), and the first five natural frequencies are obtained. By comparing the natural frequencies under different designs, effect of the hole structure on vibration characteristics of the baseplate is analyzed. The analysis results show that, compared to the solid chassis, the natural frequencies of the perforated chassis exhibit an increasing trend. Furthermore, as the number of holes increases, the natural frequency of the chassis gradually rises. The study demonstrates that the perforated design not only effectively reduces the mass of the chassis, achieving lightweight design, but also improves the natural frequency of the chassis, thus preventing resonance with the operating frequency. This research provides theoretical basis and reference for the optimization design of centrifuge chassis, helping to enhance the long-term stability and reliability of the equipment.