Abstract: Gene sequencers serve as indispensable cornerstone instruments in modern life science research and medical practice. As a core module of the gene sequencer, the chipholder provides stable optical fixation support for imaging the sequencing chip. Simultaneously, it delivers the precise thermal environment required for biochemical reactions within the chip, ensuring stable sequencing processes. The temperature control system, a critical component of the chipholder, employs a Thermoelectric Cooler （TEC） combined with a water-cooling heat dissipation solution. Its performance was rigorously tested and analyzed. Test results demonstrate that the temperature control system meets the stringent requirements for biochemical sequencing reactions, including: Ramp Rates with Minimal Overshoot: Heating from 20 ℃ to 70 ℃ in <30 s; Cooling from 65 ℃ to 20 ℃ in <40 s （with temperature overshoot/undershoot maintained below ±1 ℃ during transitions）. Temperature Uniformity: Overall temperature uniformity across the chip surface ≤±1 ℃. Temperature Stability: Control stability within ±0.5 ℃ upon reaching set point. It provides a valuable reference for subsequent design and development efforts, contributing significantly to advancing the domestic development of the gene sequencer industry.