Abstract: To address the problems in traditional flexographic plate replacement processes, which rely on manual pushing and pulling of the overturning seat, manual locking, and planar positioning, resulting in long replacement time and low positioning accuracy, this paper improves the design in terms of bearings, repeat positioning accuracy, and operational convenience, and proposes a printing plate replacement structure based on cylinder drive and conical surface positioning. The structure adopts high load-bearing capacity bearings and realizes the automatic separation and reset of the mandrel and the push-pull seat through the reciprocating motion of the cylinder. High-precision repeat positioning is achieved through the matching of the cone tip and the conical sleeve. The results show that the proposed structure can effectively improve positioning accuracy and replacement efficiency, reduce manual labor intensity, and enhance printing quality.